The three players did not feature in England's crushing eight-wicket defeat in Perth last week, a match that ended as the shortest Ashes test by overs bowled to produce a result since 1888.
"The England Men's Ashes Test squad have released three players – Jacob Bethell, Matthew Potts and Josh Tongue – to join the England Lions for their forthcoming two-day fixture against the Australian Prime Minister's XI in Canberra," ECB said in a statement.
The match will be a day-night fixture, played with the pink ball, starting on Saturday.
Australia head into the Brisbane test with momentum and an imposing record in day-night matches, having won 13 of their 14 tests played under lights, including all three against England.