There are several windows of opportunity to register players with the Confederation of African Football to play in continental competition and Mayo has had his paperwork sorted after he missed the narrow win over Angola’s Kabuscorp in the first round.
Chiefs play their second round, first leg tie against the club from the Democratic Republic of Congo at the TP Mazembe Stadium in Lubumbashi on Saturday.
It is also expected that Saturday’s clash will mark a first outing for Lebogang Maboe, who was signed on free transfer after being released from his contract at Mamelodi Sundowns.
The 31-year-old Maboe, whose injury woes in recent seasons have stymied his progress, last played on the final day of the previous league season in May, when he ended his loan spell at SuperSport United.
Chiefs also have Mfundo Vilakazi, back in their squad. He had been in Chile with South Africa’s under-20 team who made it past the first round but were eliminated in the last 16.
Chiefs travelled with a 22-man squad to Lubumbashi on Thursday, which included Rwanda goalkeeper Fiacre Ntwari.
He refused to come off the pitch when coach Khalid Ben Youssef was seeking to substitute him near the end oi their last match against Stellenbosch and it was expected he would face some sanction from the club.
Ntwari kept goal for his country in Nelspruit on Tuesday as they lost 3-0 to South Africa, who qualified for the World Cup.
Chiefs host the second leg of their tie against the Congolese newcomers at Dobsonville Stadium next Saturday.